i have done this:

import os
import fiona
rootdir = r'C:\Users\user\Desktop\a' # path to your root directory you walk
sfiles = {} # a dictionary with all the .shp files
for entry in os.listdir(rootdir):
    dirpath = os.path.join(rootdir, entry)
    if os.path.isdir(dirpath): 
        for file in os.listdir(dirpath): # Get all files in the subdirectories
            if file.endswith('.shp'): # If it's an .shp.
                filepath = os.path.join(dirpath, file)
                sfiles[filepath[:-4]] = fiona.open(filepath)

then you make an action dict like this:

action_dict = {'INT': 'intersection', 'ER': 'difference'}

and later

action, shape1, shape2 = line.split('_')  # here line is ER_ASTOM_ASTIK or whatever line in your txt file
if shape1 in sfiles and shape2 in sfiles:
    gpd.overlay(sfiles[shape1], sfiles[shape2], how=action_dict[action])

THE LAST VERSION

THE LAST VERSION

can you make needed corrections to make it work?

THE LAST VERSION

import os
import fiona
rootdir = r'C:\Users\user\Desktop\a' # path to your root directory you walk
sfiles = {} # a dictionary with all the .shp files
for entry in os.listdir(rootdir):
    dirpath = os.path.join(rootdir, entry)
    if os.path.isdir(dirpath): 
        for file in os.listdir(dirpath): # Get all files in the subdirectories
            if file.endswith('.shp'): # If it's an .shp.
                filepath = os.path.join(dirpath, file)
                sfiles[filepath[:-4]] = fiona.open(filepath)

action_dict = {'INT': 'intersection', 'ER': 'difference'}


directory=input('Insert dir of the main folder')
with open(input()) as txtfile: #insert directory of txt
    x = txtfile.readlines()
for line in x:
    action, shape1, shape2 = line.split('_')  # here line is ER_ASTOM_ASTIK or whatever line in your txt file
    if shape1 in sfiles and shape2 in sfiles:
        gpd.overlay(sfiles[shape1], sfiles[shape2], how=action_dict[action])

it doesn't probably perform the intersection or difference. how can i store its results in a variable because i have to do further process with the outcomes of every operation of every line.