I have a field where the user inputs text Aa1Bb2Cc3
Am I able to auto populate another field by only populating the field with Bb or 3.
Auto populate the field with characters [4:6] or just 
How would I do this?
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Yes, you should be able to do this. You can reference Survey123 Formulas
and also the Quick reference guide
The functions below should allow you auto populate another field.
Function Description Example
Checks if answer is selected. Used for select_one and select_multiple questions
Returns the number of selected answers. Used for select_multiple questions.
Returns the length of a nonempty string.
substr(question, start, end)
Returns the substring beginning at the specified start and extends to the character at index end -1, where start and end begin at 0.