I just have a problem with my Python script. I had run the Contour-tool to generate polylines.
Contour("rectExtract", "C:/fakepath/Class10.shp", 20,0)
Now I want to mark the 100 meter lines in another colour.
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As @Roy suggested, doing this using a layer file (which you can add to your map using arcpy.mapping.AddLayer) is the way to go.
To author that layer file just classify the symbology of your layer as being 100' in one colour and everything else in another. Then save that layer as a layer file.