GIS users mix spatial data layers from an early age of GI science.
What is new in the concept of "mashup"?
Is it something really new, or only a trendy word?
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A buzzword to give management something to annoy you with.
Specifically a Mashup is taking data or functionality from different sources on the web and combining them within the browser to present to the user. It is more of a Web 2.0 term than a GIS specific one. It is often used to skirt round getting proper permission to use the data to create a derivative.
On a GIS context, a mashup is the process of combining multiple sources of data into one integrated spatial display. Usually, it's about extracting spatial data from a non-spatial source and displaying it on a map.
One nice and classic example is Forbes US Demographic statistics mashup using Google maps.
Another (important!) example is missing children. One can iterate over a list of missing children, geocode it to get the coordinates of each case, and place each one on a map. This way, you can see all cases of missing children near a specific city. This can be useful, for example, for placing relevant ads in post offices and police stations.
See http://www.missingkidsmap.com/ for a live example.