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Another option is to use the materialize() method:

from qgis import processing

layer = QgsProject.instance().mapLayersByName('Langham_SPZs_v1_diss')[0]

outfn2 = "Langham_SPZs_final.shp"

layer.selectByExpression("number='2'")
layer2 = layer.materialize(QgsFeatureRequest().setFilterFids(layer.selectedFeatureIds()))

layer.selectByExpression("number='3'")
layer3 = layer.materialize(QgsFeatureRequest().setFilterFids(layer.selectedFeatureIds()))

processing.run("native:difference", {'INPUT' : layer3, 'OVERLAY' : layer2, 'OUTPUT': outfn2})

References:

• PyQGIS Developer Cookbook | 21. Cheat sheet for PyQGIS